The S-Monovette^® system

Stabilisation of cfDNA and RNA specimens

Unadulterated and stable blood samples are the basis for reliable and thus reproducible results of molecular biological analysis. The SARSTEDT S-Monovette^® system ensures that the highest standards for collecting venous blood are met. The patented aspiration system reduces the haemolysis of the specimens to a minimum.

The stabilisation performance of the SARSTEDT S-Monovettes is unique on the market:

S-Monovette^® cfDNA Exact

• Reliable sample stabilisation – up to 14 days at 4 °C – 37 °C
• No gDNA entry from nucleated cells:
  unadulterated and exact analysis results
• Lowest haemolysis – optimal sample qualities – reliable results

More about the S-Monovette^® on sarstedt.com

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Optimal stabilisation of liquid biopsy samples

The analysis of cell-free DNA (cfDNA) using a liquid biopsy is increasingly important in the early detection of transplant rejections, non-invasive prenatal tests, as well as the molecular characterisation and treatment monitoring of tumours. In order for cfDNA to be used for the analyses, a minimum concentration in the sample is required. Without stabilisation, the blood cells begin to release gDNA within a few hours. As a result, the original cfDNA portion in the plasma is masked too strongly by gDNA.
The problem is solved by the new S-Monovette^® cfDNA Exact, which stabilises the cfDNA at 4 °C to 37 °C for up to 14 days. The S-Monovette^® cfDNA Exact standardises the pre-analysis of liquid biopsy samples and guarantees excellent sample quality

Application notes

Applied Biosystems – MagMax™ Cell-Free DNA Isolation Kit (REF A29319) for Isolation of S-Monovette^® cfDNA Exact stabilized samples

MACHEREY-NAGEL – NucleoSpin Blood Kit (REF 740951.50) for Isolation of S-Monovette^® cfDNA Exact stabilized samples

MACHEREY-NAGEL – NucleoMag^® cfDNA kit (REF 744550.4) for Isolation of S-Monovette^® cfDNA Exact stabilized samples

MACHEREY-NAGEL – NucleoSnap^® cfDNA Plasma-Kit (REF 740300.50) for Isolation of S-Monovette^® cfDNA Exact stabilized samples

Zymo – Quick-cfDNA Serum & Plasma Kit (REF D4076) for Isolation of S-Monovette^® cfDNA Exact stabilized samples

Promega GmbH – Maxwell^® RSC ccfDNA LV Plasma Kit (REF AS1840) for Isolation of S-Monovette^® cfDNA Exact stabilized samples

Comparison of S-Monovette^® cfDNA Exact with two blood collection tubes for stabilization of cfDNA

S-Monovette^® RNA Exact

• Reliable sample stabilisation:
  
  • at room temperature for up to 5 days
  • refrigerated (8 °C) for up to 14 days
  • at -80 °C stable for many years
• Most reliable analysis results through consistently reliable stabilisation of different transcripts and highest RNA yields
• Significantly faster RNA isolation compared to established systems – effective time management – faster results

More about the S-Monovette^® on sarstedt.com

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Accurate results with the S-Monovette^® RNA Exact

Blood samples are an easily accessible source of the biomarker RNA, which can be used for gene expression analyses. Changes in gene expression due to illness can be detected in RNA analysis, which is why RNA is used for diagnosing infections, tumours, cancer, autoimmune diseases and metabolic diseases, making targeted treatment possible. However, its use has limitations due to the low stability of RNA as well as unnatural expression changes (e.g. induction of stress genes) after blood collection. This problem can be solved by using the S-Monovette^® RNA Exact, which makes the gene expression pattern actually present accessible for the follow-up analyses.
Using the S-Monovette^® RNA Exact ensures standardised sample collection for subsequent gene expression analyses.

Application notes

Stabilization of more than 47.000 transcripts by the SARSTEDT S-Monovette^® RNA Exact

The S-Monovette^® RNA Exact stabiliser solution maintains sample integrity over multiple freeze/thaw cycles without loss of quality

revvity - Automated RNA purification from S-Monovette RNA exact with the chemagic 360 instrument.

MACHEREY-NAGEL NucleoSpin^® RNA Blood Midi

Simultaneous extraction of mRNA and microRNA with the MagMAX™ mirVana™ Total RNA Isolation Kit from S-Monovette^® RNA Exact

Simultaneous extraction of mRNA and microRNA with the Maxwell^® CSC RNA Blood Kit from S-Monovette^® RNA Exact

Impact of RNA Stabilizing Blood Collection Tubes on Gene Expression Data Validity – A Comparison of S-Monovette^® RNA Exact, PAXgene™ Blood RNA Tubes & Tempus™ Blood RNA Tubes

Simultaneous extraction of mRNA and microRNA with Norgen Total RNA Purification Kit